CAS NO:15548-60-4 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside
CAS NO:15548-60-4 NAME:5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside MF：C14H15BrClNO6 MW：408.63 EINECS：239-603-0 APPEARANCE:White to Light yellow Crystalline powder COA:Provide Sample:Negotiable
Categories：
Life Science Chemicals
Biochemicals
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Product introduction
| PRODUCT NAME | 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside |
| CAS NO | 15548-60-4 |
| MP | 249-251 °C |
| BP | 673.9±55.0 °C(Predicted) |
| DENSITY | 1.882±0.06 g/cm3(Predicted) |
| STORAGE | Keep in dark place,Sealed in dry,Store in freezer, under -20°C |
| DELIVERY | 2-3DAYS |
| PACKAGE | According client's request |
Description of CAS NO:15548-60-4 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside
Chromogenic substrate for β-D-glucosidase, yielding a blue precipitate and contained in a chromogenic culture media for the detection of Enterococci, Streptococci, Klebsiella species, Enterobacter species and Listeria species. It is an indolyl carbohydrate that is the beta-D-glucoside of 3-hydroxy-1H-indole, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of the enzyme, beta-glucosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole; this immediately dimerises to give an intensely blue product.
5-Bromo-4-chloroindol-3-yl-beta-D-glucopyranoside CAS：15548-60-4 is a substrate that has been utilized in histochemical studies to detect specific enzyme activities in mammalian tissues. It is particularly useful for demonstrating the presence of beta-D-glucuronidase, an enzyme that plays a crucial role in the metabolism of complex carbohydrates. The compound reacts without the need for additional steps such as ferri-ferrocyanide or a final coupling step, which simplifies the detection process. The specificity of the reaction can be enhanced by the use of inhibitors like lactone, which allows for differentiation from other glycosidases
Applications of CAS NO:15548-60-4 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside
1.Kinetic Studies and Enzyme Analysis
Hydrolysis Kinetics : 5-Bromo-4-chloroindol-3-yl-beta-D-glucopyranoside's hydrolysis by almond emulsin was studied, revealing specific rate dependencies on buffer types and pH levels.
2.Histochemical Applications
Tissue β-D-glucuronidase Detection
3.Potential Therapeutic Applications
Synthesis and Antitumor Activity
4.Biochemical Research
Enzymatic Activity Studies : The compound is used to study the β-glucosidase enzyme
5.Photobromination in Organic Chemistry
Photo-Bromination Studies
The future directions of “5-Bromo-4-chloroindol-3-yl-beta-D-glucopyranoside”CAS：15548-60-4 could involve its use in further biomedical research, given its role as a substrate for the β-glucosidase enzyme . It could be used in studying enzyme kinetics, drug development, and understanding physiological processes
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